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Science 5 December 1975:
Vol. 190. no. 4218, pp. 983 - 985
DOI: 10.1126/science.1188378
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Articles

Science, Vol 190, Issue 4218, 983-985
Copyright © 1975 by American Association for the Advancement of Science

articles

"Half" membrane enrichment: verification by electron microscopy

KA Fisher

Membranes of intact erythrocytes bound to polylysine-treated glass fracture nonrandomly when covered with thin copper and frozen. Electron microscopic examination of the glass side reveals extensive areas of outer "half" membrane (B face) and of the copper side, inner "half" membrane (A face). This technique allows the ultrastructural examination of square-centimeter areas of fractured membrane and the chemical analysis of these membrane "halves".


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Distribution of transmembrane polypeptides in freeze fracture.
H. Edwards, T. Mueller, and M Morrison (1979)
Science 203, 1343-1346
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Freeze-fractured purple membrane particles: protein content.
K. Fisher and W Stoeckenius (1977)
Science 197, 72-74
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