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Science 7 November 1975:
Vol. 190. no. 4214, pp. 584 - 586
DOI: 10.1126/science.1188358
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Articles

Science, Vol 190, Issue 4214, 584-586
Copyright © 1975 by American Association for the Advancement of Science

articles

Guanidinium-CsCl density gradients for isopycnic analysis of nucleic acids

V Enea and ND Zinder

The addition of guanidinium chloride to CsCl gradients lowers the apparent density of RNA, DNA, and hybrid polymers in such a way that all three can be banded, fractionated, and analyzed in one gradient with essentially no damage to their chemical integrity or to their biological activity.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)