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Science 24 October 1975:
Vol. 190. no. 4212, pp. 389 - 390
DOI: 10.1126/science.1182043
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Articles

Science, Vol 190, Issue 4212, 389-390
Copyright © 1975 by American Association for the Advancement of Science

articles

Solubilization of a stereospecific opiate-macromolecular complex from rat brain

EJ Simon, JM Hiller, and I Edelman

A [3H]etorphine-macromolecular complex has been solubilized from rat brain synaptosomal fraction by extraction with the nonionic detergent Brij 36T. Stereospecificity of binding to this solubilized complex was demonstrated by the finding that radioactivity in the complex was virtually eliminated when binding had occurred in the presence of excess levorphanol, an active narcotic analgesic, while it was unaffected by its inactive enantiomorph dextrorphan. Bound radioactivity was dissociated by proteolytic enzymes, sulfhydryl reagents, and heat, suggesting the presence of protein. The bound solubilized macromolecular moiety may be the opiate receptor.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Antibodies to cerebroside sulfate inhibit the effects of morphine and beta-endorphin.
F. Craves, B Zalc, L Leybin, N Baumann, and H. Loh (1980)
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