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Science 25 July 1975:
Vol. 189. no. 4199, pp. 291 - 293
DOI: 10.1126/science.1145202
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Articles

Science, Vol 189, Issue 4199, 291-293
Copyright © 1975 by American Association for the Advancement of Science

articles

Nuclear protein matrix: association with newly synthesized DNA

R Berezney and DS Coffey

The residual structural framework of the cell nucleus termed the nuclear protein matrix, is associated with newly synthesized DNA in regenerating rat liver. One minute after rats are injected with [3-H] thymidine, more than 90 percent of the total tritium in nuclear DNA is associated with the matrix DNA although this DNA comprises only 25 percent of the total nuclear DNA. In contrast, the bulk DNA, 75 percent of total nuclear DNA, contains less than 8 percent of the total labeled DNA. The percentage of total labeled DNA associated with the bulk DNA increases for 30 minutes after injection and decreases correspondingly in the matrix DNA.


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