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Science 30 May 1975:
Vol. 188. no. 4191, pp. 943 - 945
DOI: 10.1126/science.1138363
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Articles

Science, Vol 188, Issue 4191, 943-945
Copyright © 1975 by American Association for the Advancement of Science

articles

Thick and thin filaments in postmitotic, mononucleated myoblasts

H Holtzer, K Strahs, J Biehl, AP Somlyo, and H Ishikawa

Addition of cytochalasin B to primary muscle cultures allows the physical separation of postmitotic myogenic cells from replicating presumptive myoblasts and replicating fibroblasts. Mononucleated, postmitotic myoblasts proceed without fusion to synthesize myosin and actin and to assemble these proteins into thick and thin filaments. Although sarcomeres oriented in tandem are not evident and A, H, and I bands are atypical in these mononucleated myoblasts, the irregularly scattered clusters of myofilaments are assembled into remarkably normal interdigitating arrays. These scattered clusters of stacked thick and thin filaments permit the cell to contact spontaneously in the presence of cytochalasin B.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Involvement of protein phosphatase-1-mediated MARCKS translocation in myogenic differentiation of embryonic muscle cells.
S. S. Kim, J. H. Kim, S.-H. Lee, S. S. Chung, O.-S. Bang, D. Park, and C. H. Chung (2002)
J. Cell Sci. 115, 2465-2473
   Abstract »    Full Text »    PDF »
Drosophila myoblast city Encodes a Conserved Protein That Is Essential for Myoblast Fusion, Dorsal Closure, and Cytoskeletal Organization.
M. R. S. Erickson, B. J. Galletta, and S. M. Abmayr (1997)
J. Cell Biol. 138, 589-603
   Abstract »    Full Text »    PDF »
Regulation of muscle differentiation: stimulation of myoblast fusion in vitro by catecholamines.
D. Curtis and R. Zalin (1981)
Science 214, 1355-1357
   Abstract »    PDF »


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