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Science 21 March 1975:
Vol. 187. no. 4181, pp. 1075 - 1076
DOI: 10.1126/science.187.4181.1075
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Articles

Human Skeletal Muscle: Properties of the "Chemically Skinned%" Fiber

Donald S. Wood 1, Jenny Zollman 1, John P. Reuben 1, and Philip W. Brandt 2

1 Laboratory of Neurophysiology, Department of Neurology, College of Physicians and Surgeons of Columbia University, New York 10032
2 Department of Anatomy, College of Physicians and Surgeons of Columbia University

A "skinning%" procedure is described for irreversibly disrupting the sarcolemmal membrane of human skeletal muscle and allowing calcium and other diffusible solutes (such as adenosine triphosphate) access to the myofilament space. Single skinned fibers give isometric tensions of about 1.5 kilograms per square centimeter when exposed to ionized calcium event after 1 to 2 weeks of storage at 5°C. For up to 5 days the preparation will sequester and, under appropriate conditions (anion substitution, caffeine addition, or magnesium withdrawal), release calciumn. The regulation of intracellular calcium distribution and the calcium-induced activation of the contractile proteins are discussed and related to the morphology of humnan fibers and to similar processes occurring on other muscle preparations.


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