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Science 14 March 1975:
Vol. 187. no. 4180, pp. 948 - 950
DOI: 10.1126/science.1096300
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Articles

Science, Vol 187, Issue 4180, 948-950
Copyright © 1975 by American Association for the Advancement of Science

articles

Immunofluorescence of mitotic spindles by using monospecific antibody against bovine brain tubulin

GM Fuller, BR Brinkley, and JM Boughter

Monospecific antibody directed against bovine brain tubulin has been purified by affinity chromatography and tested against soluble tubulin and intact microtubules of brain and mitotic apparatus. Binding of the tubulin antibody to the mitotic spindle of rat kangaroo cells was demonstrated in all stages of mitosis by indirect immunofluorescence.


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Tubulin Assembly Sites and the Organization of Microtubule Arrays in Mammalian Cells.
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Immunofluorescence localization of proteins of high molecular weight along intracellular microtubules.
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Quantitation of cytoplasmic tubulin by radioimmunoassay.
J. Morgan, L. Rodkey, and B. Spooner (1977)
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Microtubule assembly and the intracellular transport of secretory granules in pancreatic islets.
D. Pipeleers, M. Pipeleers-Marichal, and D. Kipnis (1976)
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