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Science 31 January 1975:
Vol. 187. no. 4174, pp. 353 - 355
DOI: 10.1126/science.187.4174.353
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Articles

Polypeptide Composition of Fraction 1 Protein from Parasexual Hybrid Plants in the Genus Nicotiana

S. D. Kung 1, J. C. Gray 1, S. G. Wildman 1, and P. S. Carlson 2

1 Department of Biology, Molecular Biology Institute, University of California, Los Angeles 90024
2 Department of Biology, Brookhaven National Laboratory, Upton, New York 11973

Analysis of the subunit polypeptide composition of Fraction 1 proteins gives information on the expression of both nuclear and chloroplast genomes; the large subunits of the protein are coded by chloroplast DNA, whereas the small subunits are coded by nuclear DNA. Fraction 1 protein isolated from the leaves of parasexual hybrid plants derived from the fusion of protoplasts of Nicotiana glauca and N. langsdorffii contains the small subunit polypeptides of both parent species and the large subunit polypeptides of only N. glauca. Fraction 1 protein isolated from the leaves of a hybrid plant obtained after the uptake of chloroplasts of N. suaveolens by protoplasts of white tissue of a variegating mutant of N. tabacum contains the large subunit polypeptides of both N. suaveolens and N. tabacum, as well as the small subunit polypeptides of both these species.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Tobacco fraction 1 protein: a unique genetic marker.
S Kung (1976)
Science 191, 429-434
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