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Science 31 January 1975:
Vol. 187. no. 4174, pp. 350 - 353
DOI: 10.1126/science.46123
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Articles

Science, Vol 187, Issue 4174, 350-353
Copyright © 1975 by American Association for the Advancement of Science

articles

Type C RNA tumor virus isolated from cultured human acute myelogenous leukemia cells

RE Gallagher and RC Gallo

Previously, type C RNA tumor virus-related components have been described in blood leukocytes from patients with acute myelogenous leukemia. These components, for example, reverse transcriptase, have been shown to be most closely related to those from two oncogenic subhuman primate type C viruses (woolly monkey sarcoma virus and gibbon ape leukemia virus). Now, we report the continuous production of budding type C viruses with the same characteristic reverse transcriptase by three separate culturings of leukocytes from a single bleeding from a patient with acute myelogenous leukemia. These isolations were made possible by the discovery of a source of conditioned media which sustains exponential growth of human myelogenous leukemia cells in liquid suspension culture.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
B-Cell Lymphoma Induction by Akv Murine Leukemia Viruses Harboring One or Both Copies of the Tandem Repeat in the U3 Enhancer.
J. Lovmand, A. B. Sorensen, J. Schmidt, M. Ostergaard, A. Luz, and F. S. Pedersen (1998)
J. Virol. 72, 5745-5756
   Abstract »    Full Text »    PDF »
Current Concepts of Leukemia and Lymphoma: Etiology, Pathogenesis, and Therapy.
C. W. BERARD, R. C. GALLO, E. S. JAFFE, I. GREEN, and V. T. DEVITA JR. (1976)
Ann Intern Med 85, 351-366
   Abstract »    PDF »
Specificity of naturally occurring antibody in normal gibbon serum.
T Aoki, M Liu, M. Walling, G. Bushar, P. Brandchaft, and T. Kawakami (1976)
Science 191, 1180-1183
   Abstract »    PDF »
Isolation of type C virions from a normal human fibroblast strain.
S Panem, E. Prochownik, F. Reale, and W. Kirsten (1975)
Science 189, 297-299
   Abstract »    PDF »
RNA processing and RNA tumor virus origin and evolution.
D Gillespie and R. Gallo (1975)
Science 188, 802-811
   Abstract »    PDF »
Primate type C virus p30 antigen in cells from humans with acute leukemia.
C. Sherr and G. Todaro (1975)
Science 187, 855-857
   Abstract »    PDF »


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