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Science 20 December 1974:
Vol. 186. no. 4169, pp. 1115 - 1117
DOI: 10.1126/science.186.4169.1115
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Articles

Radioimmunoassay for Human Procollagen

Mark B. Taubman 1, Burton Goldberg 1, and Charles J. Sherr 2

1 Department of Pathology, New York University Medical Cetnter, New York 10016
2 Viral Leukemia and Lymphoma Branch, National Cancer Institute, Bethesda, Maryland 20014

Rabbit antiserums were produced against the procollagen molecule secreted into the medium of cultured human skin fibroblasts. The isolated antigenic, amino terminal portion of the procollagen molecule was purified, labeled with iodine-125, and used in a radioimmunoassay which detected nanogram quantities of the same antigen. With the assay, immunologically identical molecules were detected in the culture mediumn of different strains of human fibroblasts and in normal human serums. Serumns from human cord blood contained a 12-fold higher concentration of the antigen than serums from adults, while serums other vertebrates gave reactions to incomplete cross-reactivity or non-reactivity.


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