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Science 13 December 1974:
Vol. 186. no. 4168, pp. 1040 - 1042
DOI: 10.1126/science.186.4168.1040
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Articles

Decrease in Free Cystine Content of Cultured Cystinotic Fibroblasts by Ascorbic Acid

Wolfgang A. Kroll 1 and Jerry A. Schneider 1

1 Department of Pediatrics, University of California, San Diego, La Jolla 92037

The 100-fold increase in free cystine content characteristic of cultured skin fibroblasts from patients with nephropathic cystinosis was decreased more than 50 percent by addition of L-ascorbic acid to the culture medium at concentrations of 0.29 to 2.9 millimolar. Fresh ascorbic acid must be added to the culture medium daily to produce a progressive decrease of the free cystine content of the cells over a 3-day period. Upon removal of ascorbic acid from the medium, the free cystine content returns to its initial value.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Ascorbic Acid-Induced Uricosuria: A Consequence of Megavitamin Therapy.
H. B. STEIN, A. HASAN, and I. H. FOX (1976)
Ann Intern Med 84, 385-388
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Science. ISSN 0036-8075 (print), 1095-9203 (online)