Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 13 December 1974:
Vol. 186. no. 4168, pp. 1036 - 1037
DOI: 10.1126/science.186.4168.1036
Prev | Table of Contents | Next

Articles

Electron Diffraction of Frozen, Hydrated Protein Crystals

Kenneth A. Taylor 1 and Robert M. Glaeser 1

1 Division of Medical Physics, Donner Laboratory, and Lawrence Berkeley Laboratory, University of California, Berkeley 94720

High-resolution electron diffraction patterns have been obtained from frozen, hydrated catalase crystals to demonstrate the feasibility of using a frozen specimen hydration technique. The use of frozen specimens to maintain the hydration of complex biological structures has certain advantages over previously developed liquid hydration techniques.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
From the Cover: Nanoscale magnetic resonance imaging.
C. L. Degen, M. Poggio, H. J. Mamin, C. T. Rettner, and D. Rugar (2009)
PNAS 106, 1313-1317
   Abstract »    Full Text »    PDF »
From the Cover: Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction.
X. Zhang, E. Settembre, C. Xu, P. R. Dormitzer, R. Bellamy, S. C. Harrison, and N. Grigorieff (2008)
PNAS 105, 1867-1872
   Abstract »    Full Text »    PDF »
Cryo-EM and Single Particles.
L. Wang and F. J. Sigworth (2006)
Physiology 21, 13-18
   Abstract »    Full Text »    PDF »
3D structure of eukaryotic flagella in a quiescent state revealed by cryo-electron tomography.
D. Nicastro, J. R. McIntosh, and W. Baumeister (2005)
PNAS 102, 15889-15894
   Abstract »    Full Text »    PDF »
A new twist on protein crystallization.
S. A. Darst (1998)
PNAS 95, 7848-7849
   Full Text »    PDF »
Lipid nanotubes as substrates for helical crystallization of macromolecules.
E. M. Wilson-Kubalek, R. E. Brown, H. Celia, and R. A. Milligan (1998)
PNAS 95, 8040-8045
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)