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Science 25 October 1974:
Vol. 186. no. 4161, pp. 357 - 359
DOI: 10.1126/science.186.4161.357
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Articles

Synthesis of Cell Wall Microfibrils in vitro by a "Soluble" Chitin Synthetase from Mucor rouxii

J. Ruiz-Herrera 1 and S. Bartnicki-Garcia 1

1 Department of Plant Pathology, University of California, Riverside 92502

A "soluble" form of chitin synthetase was separated from a membrane-rich fraction by exposure to the enzyme substrate (uridine diphosphate N-acetyl-D-glucosamine) and activator (N-acetyl- D-glucosamine). The solubilized enzyme catalyzed the synthesis of chitin microfibrils similar, if not identical, to those formed in vivo by the fungus. Cell wall microfibrils were thus abundantly formed in the absence of a living cell or its membranes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Eukaryot. Cell 7, 1685-1698
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Spitzenkorper Localization and Intracellular Traffic of Green Fluorescent Protein-Labeled CHS-3 and CHS-6 Chitin Synthases in Living Hyphae of Neurospora crassa.
M. Riquelme, S. Bartnicki-Garcia, J. M. Gonzalez-Prieto, E. Sanchez-Leon, J. A. Verdin-Ramos, A. Beltran-Aguilar, and M. Freitag (2007)
Eukaryot. Cell 6, 1853-1864
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Structural characterization by 13C-NMR spectroscopy of products synthesized in vitro by polysaccharide synthases using 13C-enriched glycosyl donors: application to a UDP-glucose:(1->3)-{beta}-D-glucan synthase from blackberry (Rubus fruticosus).
J. K. Fairweather, J. L. K. Him, L. Heux, H. Driguez, and V. Bulone (2004)
Glycobiology 14, 775-781
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In Vitro Versus in Vivo Cellulose Microfibrils from Plant Primary Wall Synthases: Structural Differences.
J. Lai-Kee-Him, H. Chanzy, M. Muller, J.-L. Putaux, T. Imai, and V. Bulone (2002)
J. Biol. Chem. 277, 36931-36939
   Abstract »    Full Text »    PDF »
Chitin: New facets of research.
P. Austin, C. Brine, J. Castle, and J. Zikakis (1981)
Science 212, 749-753
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Chitin synthetase distribution on the yeast plasma membrane.
A Duran, E Cabib, and B Bowers (1979)
Science 203, 363-365
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Science. ISSN 0036-8075 (print), 1095-9203 (online)