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Science 6 September 1974:
Vol. 185. no. 4154, pp. 859 - 862
DOI: 10.1126/science.185.4154.859
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Articles

Human Serum Albumin Phenotype Activation in Mouse Hepatoma-Human Leukocyte Cell Hybrids

Gretchen J. Darlington 1, Hans Peter Bernhard 1, and Frank H. Ruddle 1

1 Yale University, Kline Biology Tower, New Haven, Connecticut 06520

Murine hepatoma cells that secreted mouse serum albumin were fused with human leukocytes that did not produce albumin. The resulting hybrids secreted both mouse and human serum albumin, as demonstrated by immuno-electrophoretic techniques. The activation of the human genome suggests that mapping genes governing specialized functions in somatic cell hybrids may be accomplished by using adifferentiated human cells as a parental line.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)