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Science 5 July 1974:
Vol. 185. no. 4145, pp. 63 - 66
DOI: 10.1126/science.185.4145.63
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Articles

Brain Cells in Culture: Morphological Transformation by a Protein

Ramon Lim 1 and Katsusuke Mitsunobu 1

1 Division of Neurosurgery, Department of Biochemistry, and Brain Research Institute, University of Chicago, Chicago, Illinois 60637

One type of elmbryonic rat brain cell having an epithelioid morphology in the monolayer culture can be transformed by brain extract into cells having extensive processes resembling mature astrocytes. The transforming factor is a protein with a molecular weight of 350,000. A partially purified sample showed that it is active at a concentration as low as 1 x 10-8M. The transforming actvity is high in adult brains but low in embryonic brains and tumors of the nervous systems.


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