Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 21 June 1974:
Vol. 184. no. 4143, pp. 1288 - 1289
DOI: 10.1126/science.184.4143.1288
Prev | Table of Contents | Next

Articles

Positive Control of Transformed Phenotype in Hybrids between SV40-Transformed and Normal Human Cells

Carlo M. Croce 1 and Hilary Koprowski 1

1 Wistar Institute of Anatomy and Biology, Philadelphia. Pennsylvania

Somatic cell hybrids have been obtained between SV40-transformed Lesch-Nyhan fibroblasts, which are deficient in hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and display glucose-6-phosphate dehydrogenase A (G6PD-A) activity, and late-passage HGPRT-positive W138 human embryo fibroblasts, which display G6PD-B activity. The human-human hybrid clones, which display G6PD-A and G6PD-B and heteropolymers of the two enzyme forms, have the same growth characteristic as the SV40-transformed parental cells and behave as continuous cell lines. The SV40 tumor antigen, the gene for which has been assigned to human chromosome 7, is present in all clones examined.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Molecular Genetic Approaches to the Study of Cellular Senescence.
T.J. Goletz, J.R. Smith, and O.M. Pereira-Smith (1994)
Cold Spring Harb Symp Quant Biol 59, 59-66
   Abstract »    PDF »
Genetics of Cell Transformation by Simian Virus 40.
C. M. Croce, K. Huebner, A. J. Girardi, and H. Koprowski (1974)
Cold Spring Harb Symp Quant Biol 39, 335-343
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)