Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 17 May 1974:
Vol. 184. no. 4138, pp. 797 - 800
DOI: 10.1126/science.184.4138.797
Prev | Table of Contents | Next

Articles

Partition of Tissue Functions in Epithelia: Localization of Enzymes in " Mitochondria-Rich" Cells of Toad Urinary Bladder

Walter N. Scott 1, Victor S. Sapirstein 1, and Monroe J. Yoder 2

1 Departments of Ophthalmology and Physiology and Biophysics, Mount Sinai School of Medicine of the City University of New York, New York 10029
2 Department of Biology, New York University, New York 10003

The mucosal epithelium of the toad urinary bladder reabsorbs sodium, acidifies the urine, and is responsive to neurohypophyseal hormnones. Mucosal epithelial cells, consisting of two major morphologic cell types, "mitochondria-rich" and "granular," were removed from the bladder and separated by density gradient centrifugation. The mitochondria-rich cells contained three times as much carbonic anhydrase activity as the granular cells. Oxytocin caused a 235 percent increase in the adenosine 3',5'-monophosphate content of mitochondria-rich cells but had no effect on the granular cells. The evidence indicates that the mitochondria-rich cell, which accounts for only 15 percent of the mucosal cells, plays a major role in the mediation of sodium ion and hydrogen ion transport in the toad bladder and is a specific site of action of neurohypophyseal hormones.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The hydroosmotic response of frog urinary bladder to serosal hypertonicity is dependent on adenylate cyclase for its maintenance and affected by [Cl-]o changes.
A. T. Hanna-Mitchell and E. M. Gebruers (2006)
Am J Physiol Regulatory Integrative Comp Physiol 291, R213-R223
   Abstract »    Full Text »    PDF »
beta-Adrenergic regulation of adenosine 3',5'-monophosphate concentration in brain microvessels.
T. Herbst, M. Raichle, and J. Ferrendelli (1979)
Science 204, 330-332
   Abstract »    PDF »
Immunofluorescent localization of cyclic AMP in toad urinary bladder: possible intercellular transfer.
D. Goodman, F. Bloom, E. Battenberg, H Rasmussen, and W. Davis (1975)
Science 188, 1023-1025
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)