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Science 5 April 1974:
Vol. 184. no. 4132, pp. 77 - 78
DOI: 10.1126/science.184.4132.77
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Articles

Electron Diffraction of Wet Biological Membranes

S. W. Hui 1 and D. F. Parsons 1

1 Electron Optics Laboratory, Roswell Park Memorial Institute, Buffalo, New, York 14203

Electron diffraction patterns were obtained for the first time from single wet phospholipid bilayers and from wet human erythrocyte membranes by using a temperature-controlled electron microscope hydration stage. Selective area diffraction showed the existence of semicrystalline domains. A structural transition was observed at the transition temperature of the wet dipalmitoyl lecithin bilayer.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Direct observation of domains in wet lipid bilayers.
S. Hui and D. Parsons (1975)
Science 190, 383-384
   Abstract »    PDF »
Electron Diffraction of Frozen, Hydrated Protein Crystals.
K. A. Taylor and R. M. Glaeser (1974)
Science 186, 1036-1037
   Abstract »    PDF »
Structure of Wet Specimens in Electron Microscopy.
D. F. Parsons (1974)
Science 186, 407-414
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)