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Science 15 March 1974:
Vol. 183. no. 4129, pp. 1089 - 1091
DOI: 10.1126/science.183.4129.1089
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Articles

Radioreceptor Assay for 1agr,25-Dihydroxyvitamin D3

Peter F. Brumbaugh 1, David H. Haussler 1, Rubin Bressler 1, and Mark R. Haussler 1

1 Departments of Biochemistry and Pharmacology, College of Medicine, University of Arizona, Tucson 85724

A competitive protein binding assay with a sensitivity of 80 picograms has been developed for 1agr,25-dihydroxyvitamin D3, the hormonal form of vitamin D3. lagr,25-Dihydroxyvitamin D3 displaced tritiated hormone from a cytosol-chromatin receptor preparation isolated from chick small intestine, providing a simple assay for the hormone. The concentration of lagr, 25-dihydroxyvitamin D3 in human plasma, as determined by this assay, is approximately 6 nanograms per 100 milliliters; in patients with renal disease the concentration of this kidney-produced hormone is significantly lower.


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