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Science 8 March 1974:
Vol. 183. no. 4128, pp. 962 - 964
DOI: 10.1126/science.183.4128.962
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Articles

Imunoglobulin Structure: Amino Terminal Sequences of Mouse Myeloma Proteins That Bind Phosphorylcholine

P. Barstad 1, S. Rudikoff 2, M. Potter 2, M. Cohn 3, W. Konigsberg 4, and L. Hood 5

1 Division of Biology, California Institute of Technology, Pasadena 91109
2 National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
3 Salk Institute for Biological Studies, San Diego, California 92112
4 Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510
5 Division of Biology, California Instituite of Technology

The amino terminal sequences of five light and heavy immunoglobulin chains from myeloma proteins of the BALB/c mouse with binding activity to phosphorylcholine are presented. Except for a single substitution in position 4, all five heavy chains have identical amino terminal sequences through the first hypervariable region. Proteins which share unique (idiotypic) antigenic determinants are identical through the first hypervariable region of their light and heavy chains. Proteins with differing idiotypic determinants have light chains of differing amino acid sequence. These observations suggest that the heavy chain plays a more important role than the light chain in determining the phosphorylcholine binding site.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Origins of Antibody Diversity: Insights Gained from Amino Acid Sequence Studies of Elicited Antibodies.
E. Haber, M. N. Margolies, and L. E. Cannon (1977)
Cold Spring Harb Symp Quant Biol 41, 647-659
   Abstract »    PDF »
Primary Structural Differences in Myeloma Proteins That Bind the Same Haptens.
M. Potter, S. Rudikoff, M. Vrana, D. N. Rao, and E. B. Mushinski (1977)
Cold Spring Harb Symp Quant Biol 41, 661-666
   Abstract »    PDF »
Uniformity in a Clonal Repertoire: A Case for a Germ-line Basis of Antibody Diversity.
J. L. Claflin and S. Rudikoff (1977)
Cold Spring Harb Symp Quant Biol 41, 725-734
   Abstract »    PDF »
On the Genetic Dissection of a Specific Humoral Immune Response to {alpha}(1,3) Dextran.
W. Geckeler, B. Blomberg, C. de Preval, and M. Cohn (1977)
Cold Spring Harb Symp Quant Biol 41, 743-748
   Abstract »    PDF »


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