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Science 8 February 1974:
Vol. 183. no. 4124, pp. 540 - 542
DOI: 10.1126/science.183.4124.540
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Articles

Cerebroside Antibody Inhibits Sulfatide Synthesis and Myelination and Demyelinates in Cord Tissue Cultures

June M. Fry 1, Sulamith Weissbarth 1, Gerard M. Lehrer 1, and Murray B. Bornstein 2

1 Department of Neurology, Division of Neurochemistry, Mount Sinai School of Medicine of the City University of New York, New York 10029
2 Saul R. Korey Department of Neurology, Albert Einstein College of Medicine, and Rose F. Kennedy Center for Research in Mental Retardation and Human Development, New York 10461

Antiserum to cerebroside was prepared in rabbits by injection of cerebroside together with bovine serum albumin in complete Freund's adjuvant. When applied to cultures of embryo mouse spinal cord at explantation, this antiserum inhibited sulfatide synthesis and myelination; when applied to myelinated cultures it inhibited sulfatide synthesis and produced demyelination. Complement fixation assays also show antibody to cerebroside in serums from rabbits with experimental allergic encephalomyelitis induced by injection of whole white matter. Absorption of such serum with cerebroside abolishes the inhibiting and demyelinating activities.


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