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Science 1 February 1974:
Vol. 183. no. 4123, pp. 432 - 434
DOI: 10.1126/science.183.4123.432
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Articles

Carbonic Anhydrase and Osteoclasts: Localization by Labeled Inhibitor Autoradiography

Carol V. Gay 1 and Werner J. Mueller 2

1 Department of Biophysics, Pennsylvania State University, University Park 16802
2 Department of Poultry Science, Pennsylvania State University

Autoradiography with tritiated acetazolamide indicates that osteoclasts of the hen and chick contain concentrations of carbonic anhydrase which are similar to those in pancreatic acinar cells. Grain counts of osteoblasts and osteocytes were not different from background. Thus, a sufficient quantity of carbonic anhydrase seems to be present in osteoclasts to be of physiological importance in bone resorption.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The cell biology of osteoclast function.
H. Vaananen, H Zhao, M Mulari, and J. Halleen (2000)
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Localization of Carbonic Anhydrase in Living Osteoclasts with Bodipy 558/568-modified Acetazolamide, a Thiadiazole Carbonic Anhydrase Inhibitor.
K. D. Brubaker, F. Mao, and C. V. Gay (1999)
J. Histochem. Cytochem. 47, 545-550
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Inhibition of intravacuolar acidification by antisense RNA decreases osteoclast differentiation and bone resorption in vitro.
T Laitala-Leinonen, C Lowik, S Papapoulos, and H. Vaananen (1999)
J. Cell Sci. 112, 3657-3666
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Induction of Carbonic Anhydrase II Expression in Osteoclast Progenitors Requires Physical Contact with Stromal Cells.
D. M. Biskobing, D. Fan, X. Fan, and J. Rubin (1997)
Endocrinology 138, 4852-4857
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Characterization of the Osteoclast Ruffled Border Chloride Channel and Its Role in Bone Resorption.
P. H. Schlesinger, H. C. Blair, S. L. Teitelbaum, and J. C. Edwards (1997)
J. Biol. Chem. 272, 18636-18643
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Science. ISSN 0036-8075 (print), 1095-9203 (online)