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Science 28 December 1973:
Vol. 182. no. 4119, pp. 1352 - 1355
DOI: 10.1126/science.182.4119.1352
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Articles

Aggregation of HL-A Antigens at the Lymphocyte Surface Induced by Antiserum to beta2-Microglobulin

M. D. Poulik 1, Marietta Bernoco 2, D. Bernoco 2, and R. Ceppellinni 2

1 Department of Immunochemistry, Wayne State School of Medicine, William Beaumont Hospital, Royal Oak, Michigan 48072
2 Basel Institute for Immunology, Grenzacherstrasse 487, CH-4058 Basel, Switzerland

Rabbit antiserum to human beta2-microglobulin followed by goat antiserum to rabbit immunoglobulin induces aggregation of beta2-microglobulin at the lymphocyte surface, as shown by immunofluorescence and by acquisition of resistance to lysis by complement. This treatment also affects all HL-A antigens of the cell, which cap together with beta2-microglobulin, so that the cell becomes resistant to lysis upon addition of antibodies to HL-A in the presence of complement. These findings suggest that a physical linkage exists between these two classes of polypeptides at the surface of the living cell and is in agreement with recent biochemical data obtained by others with cell membrane preparations.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
HLA variants of cultured human lymphoid cells: evidence for mutational origin and estimation of mutation rate.
D Pious and C Soderland (1977)
Science 197, 769-771
   Abstract »    PDF »
Elevated salivary and synovial fluid beta2-microglobulin in Sjogren's syndrome and rheumatoid arthritis.
N Talal, H. Grey, N Zvaifler, J. Michalski, and T. Daniels (1975)
Science 187, 1196-1198
   Abstract »    PDF »
beta2-Microglobulin: Association with Lymphocyte Receptors.
M. L. Bach, S.-W. Huang, R. Hong, and M. D. Poulik (1973)
Science 182, 1350-1352
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)