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Science 21 December 1973:
Vol. 182. no. 4118, pp. 1268 - 1270
DOI: 10.1126/science.182.4118.1268
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Articles

Phosphate-Induced Protein Chromatography

Ronald A. Rimerman 1 and G. Wesley Hatfield 1

1 Department of Medical Microbiology, California College of Medicine, University of California, Irvine 92664

High phosphate concentration is shown to cause a large proportion of the proteins of a cell-free extract of Escherichia coli to bind to agarose columns to which L-valine is attached. With a decreasing concentration gradient of potassium phosphate, the proteins elute in relation to their solubility in concentrated ammonium sulfate. This column technique appears to provide a general tool for the purification of proteins.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Probing DNA Replication Mechanisms with the T4 Bacteriophage In Vitro System.
C. C. Liu, R. L. Burke, U. Hibner, J. Barry, and B. Alberts (1979)
Cold Spring Harb Symp Quant Biol 43, 469-487
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Science. ISSN 0036-8075 (print), 1095-9203 (online)