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Science 21 December 1973:
Vol. 182. no. 4118, pp. 1256 - 1258
DOI: 10.1126/science.182.4118.1256
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Articles

Neuron Geometry and Circuitry via the Electron Microscope: Intracellular Staining with Osmiophilic Polymer

Rhanor Gillette 1 and Bruce Pomeranz 1

1 Department of Zoology, University of Toronto, Toronto, Ontario, Canada

Intracellularly injected cobalt catalyzes the generation of an osmiumbinding polymer from 3,3'-diaminobenzidine in neurons of Aplysia californica. Excellent structural preservation of nervous tissue is coupled with easy recognition of fine branches of the injected neurons in the electron microscope. This permits detailed structural analysis of synaptology.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Neuronal mapping: a photooxidation reaction makes Lucifer yellow useful for electron microscopy.
A. Maranto (1982)
Science 217, 953-955
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Intracellularly injected cobaltous ions accumulate at synaptic densities.
C. Phillips (1980)
Science 207, 1477-1479
   Abstract »    PDF »


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