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Science 23 November 1973:
Vol. 182. no. 4114, pp. 836 - 837
DOI: 10.1126/science.182.4114.836
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Articles

Pyrimidine Starvation Induced by Adenosine in Fibroblasts and Lymphoid Cells: Role of Adenosine Deaminase

Howard Green 1 and Teh-Sheng Chan 1

1 Department of Biology, Massachusetts Institute of Technology, Cambridge 02139

In the presence of 10-4 to 10-5 molar adenosine, established cell lines of fibroblastic or lymphoid origin die of pyrimidine starvation. Less than lethal concentrations inhibit cell growth. Over a broad concentration range, the effects of adenosine are prevented by providing a suitable pyrimidine source. We suggest that the recently described immune deficiency disease associated with absence of adenosine deaminase may be the result of pyrimidine starvation induced by adenosine nucleotides in cells of the lymphoid system.


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