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Science 23 November 1973:
Vol. 182. no. 4114, pp. 825 - 827
DOI: 10.1126/science.182.4114.825
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Articles

Collagen Synthesis: Localization of Prolyl Hydroxylase in Tendon Cells Detected with Ferritin-Labeled Antibodies

Bjørn R. Olsen 1, Richard A. Berg 1, Yasuo Kishida 1, and Darwin J. Prockop 1

1 Department of Biochemistry, Rutgers Medical School, College of Medicine and Dentistry of New Jersey, Piscataway 08854

An improved procedure was employed for linking ferritin to antibodies against prolyl hydroxylase, the enzyme that synthesizes the hydroxyproline in collagen. By electron microscopy, the enzyme was then found to be localized in cisternae of the rough endoplasmic reticulum of embryonic tendon cells; this indicates that hydroxylation of proline occurs while newly synthesized polypeptides are fed into the cisternae.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
In Vivo Stimulation of De Novo Collagen Production Caused by Cross-linked Hyaluronic Acid Dermal Filler Injections in Photodamaged Human Skin.
F. Wang, L. A. Garza, S. Kang, J. Varani, J. S. Orringer, G. J. Fisher, and J. J. Voorhees (2007)
Arch Dermatol 143, 155-163
   Abstract »    Full Text »    PDF »
Intracellular aspects of the process of protein synthesis.
G Palade (1975)
Science 189, 347-358
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Science. ISSN 0036-8075 (print), 1095-9203 (online)