Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 12 October 1973:
Vol. 182. no. 4108, pp. 164 - 166
DOI: 10.1126/science.182.4108.164
Prev | Table of Contents | Next

Articles

Mutations in Immunoglobulin-Producing Moose Myeloma Cells

Reuben Baumal 1, Barbara K. Birshtein 2, Philip Coffino 2, and Matthew D. Scharff 2

1 Department of Immunology, Toronto Hospital for Sick Children, Toronto, Ontario, Canada
2 Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461

Three mouse myeloma cell lines were cloned in soft agar and screened by an antiserum overlay method for variants defective in secretion of the myeloma protein. Variants that had lost the capacity to synthesize heavy chains arose spontaneously at a high rate of about 10-3 per cell per generation. Such variants lost the capacity to produce light chains at a similarly high rate. After cells were treated with the acridine half mustard ICR-191, variants occurred with an even higher incidence, and some of these synthesized heavy chains differing from that of the parent.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Hypermutation Rate Normalized by Chronological Time.
C. L. Wang and M. Wabl (2005)
J. Immunol. 174, 5650-5654
   Abstract »    Full Text »    PDF »
The role of somatic hypermutation in the generation of antibody diversity.
D. French, R Laskov, and M. Scharff (1989)
Science 244, 1152-1157
   Abstract »    PDF »
Derivation and diversification of monoclonal antibodies.
G Kohler (1986)
Science 233, 1281-1286
   PDF »
Monoclonal antibodies reveal the structural basis of antibody diversity.
J. Teillaud, C Desaymard, A. Giusti, B Haseltine, R. Pollock, D. Yelton, D. Zack, and M. Scharff (1983)
Science 222, 721-726
   Abstract »    PDF »
Regulation of Immunoglobulin Expression in Mouse Myeloma Cells.
D. H. Margulies, W. Cieplinski, B. Dharmgrongartama, M. L. Gefter, S. L. Morrison, T. Kelly, and M. D. Scharff (1977)
Cold Spring Harb Symp Quant Biol 41, 781-791
   Abstract »    PDF »
Mutations in Mouse Myeloma Cells: Implications for Human Multiple Myeloma and the Production of Immunoglobulins.
S. WEITZMAN, D. H. MARGULIES, and M. D. SCHARFF (1976)
Ann Intern Med 85, 110-116
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)