Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 31 August 1973:
Vol. 181. no. 4102, pp. 863 - 865
DOI: 10.1126/science.181.4102.863
Prev | Table of Contents | Next

Articles

Plasma Membranes: Isolation from Naturally Fused and Lysolecithin-Treated Muscle Cells

Minocher Reporter 1 and Dan Raveed 1

1 Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45378

A medium containing bicarbonate and calcium was used to isolate plasma membranes of cultured muscle cells. Membranes from differentiated myotubes, as well as the labile, largely unfused, lysolecithin-treated cells from the same culture could be isolated by identical manipulations. Adenylate cyclase of high specific activity was assayed in plasma membranes from both types of cells. Lysolecithin treatment apparently interferes with the metabolism and turnover of membrane phospholipids and thus prevents the natural fusion of muscle cells.

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)