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Science 17 August 1973:
Vol. 181. no. 4100, pp. 676 - 678
DOI: 10.1126/science.181.4100.676
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Articles

Stimulus-Coupled Secretion of ggr-Aminobutyric Acid from Rat Brain Synaptosomes

William B. Levy 1, Dianna A. Redburn 1, and Carl W. Cotman 1

1 Department of Psychobiology, University of California, Irvine 92664

Synaptosomes treated with radioactive ggr-aminobutyric acid can be stimulated to release this substance. The release is maximal within 40 seconds after stimulation and is dependent on calcium. Magnesium and manganese ions, known to block stimulus-secretion coupling processes, depress calcium-dependent release. This release is specific to synaptosomes because microsomal or myelin fractions do not release accumulated ggr-aminobutyric acid. The data illustrate a simple in vitro system suitable for analysis of secretion of ggr-aminobutyric acid in brain and in addition describe several new aspects of uptake and secretion of this compound at brain nerve endings.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Uptake and Release of Putative Neurotransmitters: Measurements in Regions of the Normal and Newcastle Disease Virus-Infected Mouse Brain.
S. C. Bondy, J. S. Burks, and M. E. Harrington (1979)
Arch Neurol 36, 540-543
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Science. ISSN 0036-8075 (print), 1095-9203 (online)