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Science 1 June 1973:
Vol. 180. no. 4089, pp. 968 - 971
DOI: 10.1126/science.180.4089.968
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Articles

Radioreceptor Assay for Prolactin and Other Lactogenic Hormones

R. P. C. Shiu 1, P. A. Kelly 1, and H. G. Friesen 1

1 Department of Experimental Medicine and McGill University Clinic, Montreal, Quebec, Canada

A radioreceptor assay with a sensitivity of 5 nanograms per milliliter has been developed for mammalian and avian pituitary prolactin, placental lactogenic hormones, and humnan growth hormone, using a membrane receptor preparation isolated from rabbit mammary glands. Prolactin preparations inhibited the binding of [125I]prolactin to receptors in direct proportion to the biological potency of these preparations. Thus, the radioreceptor assay provides a convenient and simnple assay for hormones which have lactogenic activity.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Induction of Relaxin Messenger RNA Expression in Response to Prolactin Receptor Activation Requires Protein Kinase C {delta} Signaling.
C. A. Peters, E. T. Maizels, M. C. Robertson, R. P.C. Shiu, M. S. Soloff, and M. Hunzicker-Dunn (2000)
Mol. Endocrinol. 14, 576-590
   Abstract »    Full Text »
Mechanisms Controlling the Function and Life Span of the Corpus Luteum.
G. D. Niswender, J. L. Juengel, P. J. Silva, M. K. Rollyson, and E. W. McIntush (2000)
Physiol Rev 80, 1-29
   Abstract »    Full Text »    PDF »
Prolactin receptors in Rana catesbeiana during development and metamorphosis.
B. White and C. Nicoll (1979)
Science 204, 851-853
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Prolactin synthesis by human chorion-decidual tissue: a possible source of prolactin in the amniotic fluid.
A Golander, T Hurley, J Barrett, A Hizi, and S Handwerger (1978)
Science 202, 311-313
   Abstract »    PDF »
Pituitary Tumors and Hyperprolactinemia.
W. B. Malarkey and J. C. Johnson (1976)
Arch Intern Med 136, 40-44
   Abstract »    PDF »
Prolactin Receptors in an Estrogen Receptor-Deficient Mammary Carcinoma.
M. E. Costlow, R. A. Buschow, and W. L. McGuiri (1974)
Science 184, 85-86
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