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Science 20 April 1973:
Vol. 180. no. 4083, pp. 304 - 306
DOI: 10.1126/science.180.4083.304
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Articles

Cyclic AMP Phosphodiesterase in Cloned Astrocytoma Cells: Norepinephrine Induces a Specific Enzyme Form

Petko Uzunov 1, Harvey M. Shein 2, and Benjamin Weiss 3

1 Laboratory of Preclinical Pharmacology, National Institute of Mental Health, Saint Elizabeths Hospital, Washington, D.C. 20032
2 McLean Hospital, Belmont, Massachusetts 02178
3 Laboratory of Preclinical Pharomacology, National Institute of Mental Health

The soluble supernatant fraction of homogenates of cloned rat astrocytoma cells (linle C-2A) was subjected to polyacrylamide gel electrophoresis. Two peaks of adenosine 3',5'-monophosphate phosphodiesterase activity were found, corresponding to peaks I and IV of a similarly prepared homogenate of rat brain. Incubating cells with norepinephrine (0.3 mullimolar) caused about a threefold increase in the activity of peak IV but no change in peak I. This increase was completely inhibited by prior inclubation with propranolol (0.1 millimnolar), a beta-adrenergic blocking agent, or with cyclohexamnine (40 micromolar). a protein synthesis inhibitor. Induction of a specific phosphodiesterase form by norepinephrine sitggests another feedback control mechanism whereby an organism can prevent the effects of excessive sympathetic activity.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Reversal of Catecholamine Refractoriness by Inhibitors of RNA and Protein Synthesis.
J. de Vellis and G. Brooker (1974)
Science 186, 1221-1223
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