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Science 20 April 1973:
Vol. 180. no. 4083, pp. 296 - 298
DOI: 10.1126/science.180.4083.296

Articles

Lignification in Trees: Indication of Exclusive Peroxidase Participation

John M. Harkin 1 and John R. Obst 1

1 Forest Products Laboratory, Forest Service, U.S. Department of Agriculture, Madison, Wisconsin 53705

Syringaldazine did not turn purple on cross sections of tree branches or saplings or on cambial tissue cultures unless hydrogen peroxide was added; this indicated the absence of laccase but presence of peroxidase in lignifying cells. Peroxidase, therefore, apparently is the only en enzyme that polymerizes p-coumaryl alcohols to lignin in trees.


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