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Science 16 March 1973:
Vol. 179. no. 4078, pp. 1136 - 1139
DOI: 10.1126/science.179.4078.1136
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Articles

Detection of Inborn Errors of Metabolism: Galactosemia

Helene Z. Hill 1 and Theodore T. Puck 1

1 Department of Biophysics and Genetics, University of Colorado Medical Center, Denver 80220

Radioautography of cultured, human, galactosemic and nongalactosemic cells shows that, in the presence of 0.05M D-galactono-ggr-lactone, the former incorporate much less galactose in acid-insoluble form than the latter. Presumably the lactone inhibits incorporation of the labeled galactose into pathways which do not require galactose-1-phosphate uridylyltransferase activity. Definite differences between the galactosemic and nongalactosemic condition can be demonstrated with as few as 100 to 1000 cells. This approach may be useful in facilitating prenatal detection of several kinds of inborn errors of metabolism.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Galactosemia and Galactonolactone: Further Biochemical Observations.
T. B. Friedman, R. J. Yarkin, C. R. Merril, H. Z. Hill, and C.-Y. Young (1974)
Science 183, 764-766
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Science. ISSN 0036-8075 (print), 1095-9203 (online)