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Science 12 January 1973:
Vol. 179. no. 4069, pp. 187 - 189
DOI: 10.1126/science.179.4069.187
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Articles

Isolation of Aleutian Mink Disease Virus by Affinity Chromatography

A. J. Kenyon 1, J. E. Gander 2, C. Lopez 3, and R. A. Good 3

1 Department of Pathology, University of Minnesota Medical School, Minneapolis 55455
2 Department of Biochemistry, College of Biological Sciences, University of Minnesota, Minneapolis 55455
3 Department of Pathology, University of Minnesota Medical School

Affinity chromatography was used to isolate the Aleutian disease virus of mink. Dissociation of the immunoadsorbent-virus complex with 0.75 molar sodium chloride and then with a glycine-hydrochloride gradient released infective particles resembling picornaviruses. The elution profile suggests that two different types of virus-antibody complexes are formed, one dissociated by sodium chloride and another that requires glycine-hydrochloride in addition to sodium chloride for release of virus.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)