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Science 24 November 1972:
Vol. 178. no. 4063, pp. 871 - 872
DOI: 10.1126/science.178.4063.871
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Articles

Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range

Sidney Udenfriend 1, Stanley Stein 1, Peter Böhlen 1, Wallace Dairman 1, Willy Leimgruber 2, and Manfred Weigele 2

1 Roche Institute of Molecular Biology, Nutley, New Jersey 07110
2 Chemical Research Department, Hoffmann-La Roche Inc., Nutley, New Jersey 07110

Fluorescamine is a new reagent for the detection of primary amines in the picomole range. Its reaction with amines is almost instantaneous at room temperature in aqueous media. The products are highly fluorescent, whereas the reagent and its degradation products are nonfluorescent. Applications are discussed.


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