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Science 4 August 1972:
Vol. 177. no. 4047, pp. 436 - 438
DOI: 10.1126/science.177.4047.436
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Articles

Adenosine 3',5'-Monophosphate Is Localized in Cerebellar Neurons: Immunofluorescence Evidence

F. E. Bloom 1, B. J. Hoffer 1, E. R. Battenberg 1, G. R. Siggins 1, A. L. Steiner 2, C. W. Parker 3, and H. J. Wedner 3

1 Laboratory of Neuropharmacology, Division of Special Mental Health Research, National Institute of Mental Health, Saint Elizabeths Hospital, Washington, D.C. 20032
2 Department of Medicine, Albany Medical College, Albany, New York
3 Division of Immunology, Department of Medicine, Washington University Medical School, Saint Louis, Missouri 63110

Adenosine 3',5'-monophosphate is localized in specific cerebellar neurons, as shown by fluorescence immunocytochemistry with a specific rabbit immunoglobulin. Positive staining is exhibited by Purkinje neurons and granule cells. The increase in concentration of cyclic adenosine monophosphate in the cerebellum, which is known to follow decapitation, is represented by greatly increased fluorescence of Purkinje neurons only. These immunofluorescence data provide the first evidence for localization of cyclic adenosine monophosphate in specific neurons and may permit further exploration into the role of this cyclic nucleotide in neuronal function.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Noradrenergic Stimulation of Cyclic Adenosine Monophosphate in Rat Purkinje Neurons: An Immunocytochemical Study.
G. R. Siggins, E. F. Battenberg, B. J. Hoffer, F. E. Bloom, and A. L. Steiner (1973)
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