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Science 4 August 1972:
Vol. 177. no. 4047, pp. 429 - 431
DOI: 10.1126/science.177.4047.429
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Articles

An Isomorphous Heavy-Atom Derivative of Crystalline Formylmethionine Transfer RNA

R. W. Schevitz 1, M. A. Navia 1, D. A. Bantz 1, G. Cornick 1, J. J. Rosa 1, M. D. H. Rosa 1, and P. B. Sigler 1

1 Department of Biophysics, University of Chicago, Chicago, Illinois 60637

An isomorphous osmium derivative of crystalline yeast initiator transfer RNA has been prepared and interpreted to 6-angstrom resolution. The coordinates of the heavy atoms have been determined by Patterson and "direct" methods applied to the difference coefficients of the centric projections, followed by least-squares refinement. There is one dominant site per asymmetric unit, consistent with the finding by neutron-activation analysis that there is approximately one osmium atom per molecule of transfer RNA. The osmium derivative appears to be a normal substrate for enzymatic aminoacylation.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)