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Science 21 July 1972:
Vol. 177. no. 4045, pp. 276 - 279
DOI: 10.1126/science.177.4045.276
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Articles

Terminal Saccharides on Sperm Plasma Membranes: Identification by Specific Agglutinins

Garth L. Nicolson 1 and Ryuzo Yanagimachi 2

1 Cancer Council Laboratory, Armand Hammer Cancer Center, Salk Institute for Biological Studies, San Diego, California 92112
2 Department of Anatomy, University of Hawaii School of Medicine, Honolulu 96822

Six specific agglutinins were used to identify the terminal sugar residues in the surface oligosaccharides of rabbit and hamster spermatozoa by specific agglutination. Species differences in epididymal sperm were found in the terminal residues, resembling agr-D-mannose, D-galactose, N-acetyl-D-glucosamine, and N-acetyl-D-galactosamine. Species similarities were found in terminal residues, resembling L-fucose and N-acetylneuraminic acid. When ejaculated rabbit sperm were compared to epididyimal sperm, the latter were more agglutinable with a specific agglutinin recognizing N-acetyl-D-glucosamine.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Evidence for the involvement of PECAM-1 in a receptor mediated signal-transduction pathway regulating capacitation-associated tyrosine phosphorylation in human spermatozoa.
B. Nixon, J. W. Paul, C. M. Spiller, A. G. Attwell-Heap, L. K. Ashman, and R. J. Aitken (2005)
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Mobility and the Restriction of Mobility of Plasma Membrane Lectin-Binding Components.
G. L. Nicolson and R. Yanagimachi (1974)
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Science. ISSN 0036-8075 (print), 1095-9203 (online)