Indole-3-Acetic Acid in-Human Cerebrospinal Fluid: Identification and Quantification by Mass Fragmentography
Leif Bertilsson 1 and
Lena Palmér 2
1 Departments of Pharmacology (Division of Clinical Pharmacology), Toxicology (Swedish Medical Research Council), and Psychiatry (at Karolinska Hospital), Karolinska Institutet, S-104 01 Stockholm 60, Sweden
2 Department of Toxicology, Swedish Medical Research Council, Karolinska Institutet
Indole-3-acetic acid has been identified in human cerebrospinal fluid by the gas chromatographic-mass spectrometric technique called mass fragmentography. A specific and sensitive method for quantitative determination of indole-3-acetic acid down to 2 nanograms per milliliter of cerebrospinal fluid has been developed. Samples of cerebrospinal fluid from 24 patients with depression contained 6.1 ± 3.1 (range 2.6 to 15.8) nanograms of indole-3-acetic acid per milliliter.
