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Science 9 June 1972:
Vol. 176. no. 4039, pp. 1143 - 1145
DOI: 10.1126/science.176.4039.1143
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Articles

Morphine-3-Succinyl—Bovine Serum Albumin: An Immunogenic Hapten-Protein Conjugate

Bruce H. Wainer 1, Frank W. Fitch 1, Richard M. Rothberg 2, and Josef Fried 3

1 Department of Pathology, University of Chicago, Chicago, Illinois 60637
2 Department of Pediatrics, University of Chicago
3 Departments of Biochemistry and Chemistry and Ben May Laboratory, University of Chicago

Morphine-3-hemisuccinate was synthesized by reaction of morphine with succinic anhydride. This compound was conjugated to bovine serum albumin by the mixed anhydride method, and the degree of conjugation was determined by base hydrolysis of the conjugate, extraction, and measurement of free morphine. An average of 6.5 molecules of morphine were conjugated to each molecule of protein. Eleven rabbits immunized with varying doses of the conjugate were producing antibody 8 weeks later, as determined by a modification of the ammonium sulfate method, which measures primary binding of antigen by antibody.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Immunochemical and Biologic Properties of Antibodies against Opioids.
B. H. Wainer, K. Bonese, F. W. Fitch, and R. M. Rothberg (1977)
Toxicol Pathol 5, 5-10
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Science. ISSN 0036-8075 (print), 1095-9203 (online)