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Science 9 June 1972:
Vol. 176. no. 4039, pp. 1127 - 1129
DOI: 10.1126/science.176.4039.1127
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Articles

Calcium Transient in Presynaptic Terminal of Auid Giant Synapse: Detection with Aequorin

R. Llinás 1, J. R. Blinks 1, and C. Nicholson 1

1 Division of Neurobiology, Department of Physiology and Biophysics, University of Iowa, Iowa City 52240, and Department of Pharmacology, Mayo Medical School, Rochester, Minnesota 55901

Microinjection of aequorin, a bioluminescent protein sensitive tocalcium, into the presynaptic terminal of the squid giant synapse demnonstrated an increase in intracellular calcium ion concentration during repetitive synaptic transmission. Although no light flashes synchronous with individual presynaptic : tion potentials were detected, the results are considered consistent with the hypothesis that entry of calcium into the presynaptic terminal triggers release of e synaptic transmitter substance.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)