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Science 2 June 1972:
Vol. 176. no. 4038, pp. 1027 - 1029
DOI: 10.1126/science.176.4038.1027
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Articles

Specific Inhibition of Plaque Formation to Phosphorylcholine by Antibody against Antibody

Humberto Cosenza 1 and Heinz Köhler 1

1 Department of Pathology, University of Chicago, Chicago, Illinois 60637

Spleen cells from BALB/c mice immunized with heat-killed rough pneumococci (strain R36A) or spleen cells from normal mice immunized in vitro with the same antigen produce direct hemolytic plaques against sheep erythrocytes coated with pneumococcal C polysaccharide or conjugated with phosphorylcholine. Formation of plaques is specifically inhibited by phosphorylcholine or by antiserum to mouse immunoglobulin A myeloma protein which binds phosphorylcholine. Thus, the myeloma proteins and normal BALB/c antibodies share similar idiotypic determinants. This experimental system is suitable for probing the role of the antigen receptor in the immune response.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
T15-Idiotype-Negative B Cells Dominate the Phosphocholine Binding Cells in the Preimmune Repertoire of T15i Knockin Mice.
L. Hu, L. J. Rezanka, Q.-S. Mi, A. Lustig, D. D. Taub, D. L. Longo, and J. J. Kenny (2002)
J. Immunol. 168, 1273-1280
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Antibodies to the FVIII light chain that neutralize FVIII procoagulant activity are present in plasma of nonresponder patients with severe hemophilia A and in normal polyclonal human IgG.
A. Moreau, S. Lacroix-Desmazes, N. Stieltjes, E. Saenko, S. V. Kaveri, R. D'Oiron, Y. Sultan, D. Scandella, and M. D. Kazatchkine (2000)
Blood 95, 3435-3441
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Autoreactive B Cells Escape Clonal Deletion by Expressing Multiple Antigen Receptors.
J. J. Kenny, L. J. Rezanka, A. Lustig, R. T. Fischer, J. Yoder, S. Marshall, and D. L. Longo (2000)
J. Immunol. 164, 4111-4119
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Origin and Differentiation of Lymphocytes Involved in the Secretory IgA Response.
J. J. Cebra, P. J. Gearhart, R. Kamat, S. M. Robertson, and J. Tseng (1977)
Cold Spring Harb Symp Quant Biol 41, 201-215
   Abstract »    PDF »
Primary Structural Differences in Myeloma Proteins That Bind the Same Haptens.
M. Potter, S. Rudikoff, M. Vrana, D. N. Rao, and E. B. Mushinski (1977)
Cold Spring Harb Symp Quant Biol 41, 661-666
   Abstract »    PDF »
Structure and Immunosuppression of a Cross-reactive Idiotype Associated with Anti-p-azophenylarsonate Antibodies of Strain-A Mice.
S.-T. Ju, F. L. Owen, and A. Nisonoff (1977)
Cold Spring Harb Symp Quant Biol 41, 699-707
   Abstract »    PDF »
Idiotypes and Anti-idiotypes as Probes in Analysis of Immunoregulation.
H. Cosenza, A. A. Augustin, and M. H. Julius (1977)
Cold Spring Harb Symp Quant Biol 41, 709-718
   Abstract »    PDF »
Immune Responses of BALB/c Mice to the Idiotype of T15 and of Other Myeloma Proteins of BALB/c Origin: Implications for an Immune Network and Antibody Multispecificity.
N. Sakato, C. A. Janeway Jr., and H. N. Eisen (1977)
Cold Spring Harb Symp Quant Biol 41, 719-724
   Abstract »    PDF »
Uniformity in a Clonal Repertoire: A Case for a Germ-line Basis of Antibody Diversity.
J. L. Claflin and S. Rudikoff (1977)
Cold Spring Harb Symp Quant Biol 41, 725-734
   Abstract »    PDF »
Neonatal Tolerance Induced by Antibody against Antigen-Specific Receptor.
D. S. Strayer, H. Cosenza, W. M. F. Lee, D. A. Rowley, and H. Kohler (1974)
Science 186, 640-643
   Abstract »    PDF »
Clonal Depletion in Neonatal Tolerance.
H. Kohler, D. R. Kaplan, and D. S. Strayer (1974)
Science 186, 643-644
   Abstract »    PDF »
Specific Suppression of Immune Responses.
D. A. Rowley, F. W. Fitch, F. P. Stuart, H. Kohler, and H. Cosenza (1973)
Science 181, 1133-1141
   Abstract »    PDF »
Highly reduced protection against Streptococcus pneumoniae after deletion of a single heavy chain gene in mouse.
Q.-S. Mi, L. Zhou, D. H. Schulze, R. T. Fischer, A. Lustig, L. J. Rezanka, D. M. Donovan, D. L. Longo, and J. J. Kenny (2000)
PNAS 97, 6031-6036
   Abstract »    Full Text »    PDF »


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