Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 28 April 1972:
Vol. 176. no. 4033, pp. 420 - 422
DOI: 10.1126/science.176.4033.420
Prev | Table of Contents | Next

Articles

Interactions of Immunoglobulins G and M in the Detection of the Mammalian C-Type Virus Cross-Reactive Antigen

Stephen Oroszlan 1, David Bova 1, Robert Toni 1, and Raymond V. Gilden 1

1 Flow Laboratories, Rockville, Maryland 20852

The mammalian C-type tumor viruses share an antigenic determinant, gs-3, located on the major internal polypeptide of the virion. Detection of this determined in gel diffusion assays by antiserums prepared in rats by immunization with rat tumor homogenates carrying murine virus and serums prepared in a rabbit by immunization with purified murine gs antigen depended on antibodies present in the fractions containing immunoglobulins M and G. The immunoglobulin G fraction by itself precipitated only the homologous murine antigen. Neither fraction alone precipitated heterologous (cat, rat, or hamster) antigen (definition of the gs-3 reaction), while a mixture of the two fractions did. The gs-3 reaction was eliminated by treatment of the serums with beta-mercaptoethanol, also indicating a requirement for immunoglobulin M antibodies.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Angiokeratoma Corporis Diffusum Associated With {beta}-Mannosidase Deficiency.
M. Rodriguez-Serna, R. Botella-Estrada, A. Chabas, M.-J. Coll, V. Oliver, M.-I. Febrer, and A. Aliaga (1996)
Arch Dermatol 132, 1219-1222
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)