Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 28 April 1972:
Vol. 176. no. 4033, pp. 412 - 414
DOI: 10.1126/science.176.4033.412
Prev | Table of Contents | Next

Articles

Branching of Central Neurons: Intracellular Cobalt Injection for Light and Electron Microscopy

Robert M. Pitman 1, Charles D. Tweedle 1, and Melvin J. Cohen 1

1 Department of Biology, Yale University, New Haven, Connecticut

Cobalt chloride can be injected into an identified nerve cell body in an insect ganglion and reacted with ammonium sulfide to stain the soma and its branches with a black precipitate. The stained cell body and its branches throughout the neuropil are visible in both the light and electron microscope. In whole mount preparations, the resolution of neurites within the neuropil is of a quality that permits the comparison of branching patterns between cells and during various functional states.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Loss of Escape-Related Giant Neurons in a Spiny Lobster, Panulirus argus.
S. Y. Espinoza, L. Breen, N. Varghese, and Z. Faulkes (2006)
Biol. Bull. 211, 223-231
   Abstract »    Full Text »    PDF »
Neuronal mapping: a photooxidation reaction makes Lucifer yellow useful for electron microscopy.
A. Maranto (1982)
Science 217, 953-955
   Abstract »    PDF »
Spiking local interneurons mediate local reflexes.
M Burrows and M. Siegler (1982)
Science 217, 650-652
   Abstract »    PDF »
Impulse-coded and analog signaling in single mechanoreceptor neurons.
Y. Pasztor and B. Bush (1982)
Science 215, 1635-1637
   Abstract »    PDF »
Membrane conductance and action potential of a regenerating axonal tip.
H Meiri, M. Spira, and I Parnas (1981)
Science 211, 709-712
   Abstract »    PDF »
Intracellularly injected cobaltous ions accumulate at synaptic densities.
C. Phillips (1980)
Science 207, 1477-1479
   Abstract »    PDF »
Synaptic potentials effect the release of transmitter from locust nonspiking interneurons.
M Burrows (1979)
Science 204, 81-83
   Abstract »    PDF »
Early eye removal produces excessive bilateral branching in the rat: application of cobalt filling method.
T. Cunningham (1976)
Science 194, 857-859
   Abstract »    PDF »
Dendritic reorganization of an identified motoneuron during metamorphosis of the tobacco hornworm moth.
J. Truman and S. Reiss (1976)
Science 192, 477-479
   Abstract »    PDF »
Tracing axons and axon collaterals of spinal neurons using intracellular injection of horseradish peroxidase.
P. Snow, P. Rose, and A. Brown (1976)
Science 191, 312-313
   Abstract »    PDF »
Procion Brown: An Intracellular Dye for Light and Electron Microscopy.
B. N. Christensen (1973)
Science 182, 1255-1256
   Abstract »    PDF »
Neuron Geometry and Circuitry via the Electron Microscope: Intracellular Staining with Osmiophilic Polymer.
R. Gillette and B. Pomeranz (1973)
Science 182, 1256-1258
   Abstract »    PDF »
Connectivity Patterns of Crayfish Giant Interneurons: Visualization of Synaptic Regions with Cobalt Dye.
J. E. Mittenthal and J. J. Wine (1973)
Science 179, 182-184
   Abstract »    PDF »
Electrical Responses of Insect Central Neurons: Augmentation by Nerve Section or Colchicine.
R. M. Pitman, C. D. Tweedle, and M. J. Cohen (1972)
Science 178, 507-509
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)