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Science 28 April 1972:
Vol. 176. no. 4033, pp. 407 - 410
DOI: 10.1126/science.176.4033.407
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Articles

Radioautography of the Optic Tectum of the Goldfish after Intraocular Injection of [3H]Proline

J. H. Neale 1, E. A. Neale 1, and B. W. Agranoff 1

1 Neurosciences Laboratory, Mental Health Research Institute, University of Michigan, Ann Arbor 48104

Radioautography of the optic tectum of the goldfish, performed after injection of [3H]proline into the contralateral eye, effectively resolves several distinct layers of retinal synapses. Silver grains are found unilaterally over nerve tracts containing efferent fibers from the tectum, a result that suggests intercellular migration of labeled molecules. The low background and high specific grain density obtained with [3H]proline radioautography indicate the usefulness of this technique for the elucidation of neuroanatomical connections in the visual system.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Physiological Chemistry of the Eye.
G. W. Barber (1973)
Arch Ophthalmol 89, 236-255
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