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Science 18 February 1972:
Vol. 175. no. 4023, pp. 781 - 782
DOI: 10.1126/science.175.4023.781
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Articles

Improved Flow Rates with Porous Sephadex Gels

David H. Sachs 1 and Elizabeth Painter 2

1 Laboratory of Chemical Biology, National Institute of Arthritis anid Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014
2 Transplantation Unit, General Surgical Services, Massachusetts General Hospital, Boston 02114

The use of an internal support of siliconized glass beads 6 millimeters in diameter was found to improve markedly the flow rates obtainable with porous Sephadex gels without significant alteration of other properties of the gels. The method may be generally applicable in situations where rapid separations on Sephadex are required.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)