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Science 17 December 1971:
Vol. 174. no. 4015, pp. 1242 - 1245
DOI: 10.1126/science.174.4015.1242
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Articles

Isolation of Filaments from Brain

Michael L. Shelanski 1, Stewart Albert 1, George H. DeVries 1, and William T. Norton 1

1 Department of Pathology and Saul R. Korey Department of Neurology, Albert Einstein College of Medicine, Bronx, New York 10461

A method is presented for the isolation of filaments of 90-angstrom diameter from the white matter of bovine brain by first floating the myelinated axons in a centrifugal field and then fractionating the axons on a series of density gradients. This results in a fraction that contains two types of bundles of filaments but few other constituents. The filaments are stable over a wide range of temperatures and at both low and high ionic strength. Their density and their resistance to digestion by ribonuclease and deoxyribonuclease indicate that they are primarily protein. The molecular weight of the subunit is approximately 60,000. The protein does not comigrate with microtubule protein and does not bind cholcicine or nucleotides.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Approaches to Potential Sliding Mechanisms of Cytoplasmic Microtubules.
P. Satir (1982)
Cold Spring Harb Symp Quant Biol 46, 285-292
   Abstract »    PDF »
Chemistry and Biology of Neuronal and Glial Intermediate Filaments.
R. K. H. Liem, C. H. Keith, J. F. Leterrier, E. Trenkner, and M. L. Shelanski (1982)
Cold Spring Harb Symp Quant Biol 46, 341-350
   Abstract »    PDF »
Axons:Isolation from Mammalian Central Nervous System.
G. H. DeVries, W. T. Norton, and C. S. Raine (1972)
Science 175, 1370-1372
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)