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Science 17 December 1971:
Vol. 174. no. 4015, pp. 1233 - 1236
DOI: 10.1126/science.174.4015.1233
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Articles

Nuclear Acidic Protein Changes during Differentiation in Physarum polycephalum

Wallace M. LeStourgeon 1 and Harold P. Rusch 1

1 McArdle Laboratory for Cancer Research, Medical Center, University of Wisconsin, Madison 53706

A class of acidic nuclear phosphoproteins has been isolated throughout the mitotic cycle and at two points during differentiation in the slime mold Physarum polycephalum. The electrophoretic profiles of these proteins are reproducible and unchanging throughout the mitotic cycle, but reproducible changes occur during differentiation. The proteins are rapidly synthesized after mitosis, and their molecular weights range from 34,000 to 88,000. The proteins rapidly incorporate [32P]orthophosphate, and the content of alkali-labile phosphate increases 20 percent during the period after DNA synthesis. The proteins comprise 6.5 percent by dry weight of nuclear material while the DNA comprises about 5.5 percent. These acidic nuclear proteins may have a role in control of gene activity.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Packaging Proteins of Core hnRNP Particles and the Maintenance of Proliferative Cell States.
W. M. LeStourgeon, A. L. Beyer, M. E. Christensen, B. W. Walker, S. M. Poupore, and L. P. Daniels (1978)
Cold Spring Harb Symp Quant Biol 42, 885-898
   Abstract »    PDF »
Phosphorylation of DNA-binding Nuclear Acidic Proteins and Gene Activation in the HeLa Cell Cycle.
V. G. Allfrey, A. Inoue, J. Karn, E. M. Johnson, and G. Vidali (1974)
Cold Spring Harb Symp Quant Biol 38, 785-801
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)