Barbiturates: Radioimmunoassay
Sydney Spector 1 and
Edward J. Flynn 1
1 Department of Physiological Chemistry, Pharmacology Section, Roche Institute of Molecular Biology, Nutley, New Jersey 07110
The development of a radioimmunoassay for barbiturate is described. The barbiturate is made antigenic by coupling it to a protein, bovine gamma globulin. The radioimmunoassay can measure as little as 5 nanograms of barbiturate.